Herein, a
novel nanobiocomposite scaffold based on modifying synthesized
cross-linked terephthaloyl thiourea-chitosan hydrogel (CTT-CS hydrogel)
substrate using the extracted silk fibroin (SF) biopolymer and prepared
Mg(OH)2 nanoparticles was designed and synthesized. The
biological capacity of this nanobiocomposite scaffold was evaluated by
cell viability method, red blood cells hemolytic and anti-biofilm
assays. According to the obtained results from 3 and 7 days, the cell
viability of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold was
accompanied by a considerable increment from 62.5 to 89.6% respectively.
Furthermore, its low hemolytic effect (4.5%), and as well, the high
anti-biofilm activity and prevention of the P. aeruginosa biofilm
formation confirmed its promising hemocompatibility and antibacterial
activity. Apart from the cell viability, blood biocompatibility, and
antibacterial activity of CTT-CS/SF/Mg(OH)2 nanobiocomposite
scaffold, its structural features were characterized using spectral and
analytical techniques (FT-IR, EDX, FE-SEM and TG). As well as, given the
mechanical tests, it was indicated that the addition of SF and Mg(OH)2 nanoparticles to the CTT-CS hydrogel could improve its compressive strength from 65.42 to 649.56 kPa.