10/07/1402
Parvovirus B19 affects thrombopoietin and IL-11 gene expression in human bone marrow mesenchymal stem cells
Aim: Human bone marrow mesenchymal stem cells (hBMSCs) may be
infected by parvovirus B19 (B19V). hBMSCs support bone marrow
hematopoiesis by producing stromal cells, secretion of cytokines and
growth factors, etc. Because of the lifetime persistent infection of the
virus in healthy individual’s bone marrow, this study aims to evaluate
B19V effects on hBMSCs gene expression of some crucial hematopoietic
cytokines. Materials & methods: hBMSCs were transfected with
pHI0 plasmid containing the B19V genome. The quantitative mRNA
expression of target genes was evaluated 24 h after transfection. Results: Our findings demonstrated a significant increase in expression levels of IL-11 and TPO (p < 0.05). Conclusion:
We concluded that alteration in the gene expressions in B19V-infected
hBMSCs might have significant effects on the bone marrow
microenvironment as well as hematopoiesis.