C-type lectin (CTL) is the main protein part of the secretory-excretory product, secreted by secondary stage Toxocara canis larvae. Considering the antigenic characteristics of the protein, overlapping regions were identified as the highly potent MHC-I and MHC-II binding epitopes. Furthermore, several distinct regions were distinguished as the linear and non-linear B cell epitopes. To use this protein as a candidate for serological diagnosis, the recombinant pET-32a(+) plasmid containing the 660 bp sequence of Toxocara canis C-type lectin gene was synthesized and successfully cloned and expressed in Escherichia coli (DE3+). Subsequently, the expression of the insert was confirmed by SDS-PAGE and Western blot using an anti-His monoclonal antibody. Fifty-six serum samples were collected from pet owners. Fortyfour samples were negative and twelve samples were positive performed with a commercial ELISA kit. The same samples were also tested using dot blot and western blot analysis prepared with recombinant CTL antigen (rCTL). Interestingly, the results achieved by dot blot and western blot analysis generated with rCTL showed that two negative samples determined by ELISA tests were positive in the mentioned tests, The other samples had comparable results as well as the ELISA test. A comparison of the test results of 56 human sera showed that the results were the same between the three test methods and the Kappa coefficient was calculated 1(p-value=7.25) and 0.9(p-value=1.29). It was suspected that the rCTL is more sensitive than the whole antigen secreted by Toxocara canis. In conclusion, this antigen could be further evaluated within the supplementary studies to improve the toxocariasis diagnostic kits.