An
electrochemical aptamer-based sensor was designed to detect tetracycline
(Tet) in chicken ham. First, polyacrylonitrile (PAN) polymer was used
to prepare electrospun PAN nanofibers. PAN nanofibers were heat treated
to form electrospun carbon nanofiber (CNF) mat. The electrospun CNF mat
electrode was modified with Au and the aptamer of Tet was placed on the
mat electrode. Raman spectroscopy and scanning electron microscopy
equipped with energy-dispersive spectroscopy were used to identify the
structure and morphology of electrospun CNFs, respectively. The behavior
of CNF mat electrode was evaluated via cyclic voltammetry. The designed
aptamer biosensor had a good repeatability, reproducibility, stability,
and specific function for the detection of Tet in chicken ham. This
biosensor was able to detect the Tet in the linear range of 1 × 10–9 M to 1 × 10–4 M with the detection limit of 1.2 × 10–10 M
and preserve about 95% of its primary response after 20 days. In
addition, the recoveries of Tet were achieved ranging from 96.92 to
104.31%.